Glucocorticoids (GC) are hormones secreted in our body in response to stress such as cortisol (in humans) and corticosterone (in rodents). Excessive exposure to GC can lead to the dysregulation of various metabolic processes. The brain is a highly sensitive organ that’s critically involved in the regulation of metabolism and homeostasis. The Neuro 2A cell line was used to examine the glucoregulatory effects of a synthetic glucocorticoid, dexamethasone (DEX) at a low and high concentration for short periods of time. At the end of the experiment, we collected the cells to evaluate proteins related involved in glucose homeostasis. We found a dose effect in the upregulation of phosphorylated glucocorticoids receptors (pGR). However, we found no significant difference in the levels of GLUT-1 nor a downstream target of the insulin receptor, pFoxO1.